Functional and molecular characterization of plant growth promoting Bacillus isolates from tomato rhizosphere.
Identifieur interne : 000031 ( Main/Exploration ); précédent : 000030; suivant : 000032Functional and molecular characterization of plant growth promoting Bacillus isolates from tomato rhizosphere.
Auteurs : Sadaf Kalam [Inde] ; Anirban Basu [Inde] ; Appa Rao Podile [Inde]Source :
- Heliyon [ 2405-8440 ] ; 2020.
Abstract
The rhizosphere offers a quintessential habitat for the microbial communities and facilitates a variety of plant-microbe interactions. Members of the genus Bacillus constitute an important group of plant growth promoting rhizobacteria (PGPR), which improve growth and yield of crops. In a total of 60 bacterial isolates from the tomato rhizosphere, 7 isolates were selected based on distinct morphological characteristics and designated as tomato rhizosphere (TRS) isolates with a number suffixed viz., TRS-1, 2, 3, 4, 5, 7, and TRS-8. All the seven isolates were Gram positive, with in vitro plant growth promoting (PGP) traits like phosphate and zinc solubilization, and also produced indoleacetic acid (IAA), phytase, siderophore, hydrogen cyanide (HCN), and 1-aminocyclopropane-1-carboxylate (ACC) deaminase, besides being antagonistic to other microbes and formed biofilm. The seven isolates belonged to the genus Bacillus as per the 16S rDNA sequence analysis. Phylogenetic tree grouped the isolates into four groups, while BOX-PCR fingerprinting allowed further differentiation of the seven isolates. The PGP activity of the isolates was measured on tomato seedlings in plant tissue culture and greenhouse assays. A significant increase in root colonization was observed over 15 days with all the isolates. Greenhouse experiments with these isolates indicated an overall increase in the growth of tomato plants, over 60 days. Isolates TRS-7 and TRS-8 were best plant growth promoters among the seven isolates, with a potential as inoculants to increase tomato productivity.
DOI: 10.1016/j.heliyon.2020.e04734
PubMed: 32904284
PubMed Central: PMC7452486
Affiliations:
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Members of the genus <i>Bacillus</i> constitute an important group of plant growth promoting rhizobacteria (PGPR), which improve growth and yield of crops. In a total of 60 bacterial isolates from the tomato rhizosphere, 7 isolates were selected based on distinct morphological characteristics and designated as tomato rhizosphere (TRS) isolates with a number suffixed <i>viz</i>., TRS-1, 2, 3, 4, 5, 7, and TRS-8. All the seven isolates were Gram positive, with <i>in vitro</i> plant growth promoting (PGP) traits like phosphate and zinc solubilization, and also produced indoleacetic acid (IAA), phytase, siderophore, hydrogen cyanide (HCN), and 1-aminocyclopropane-1-carboxylate (ACC) deaminase, besides being antagonistic to other microbes and formed biofilm. The seven isolates belonged to the genus <i>Bacillus</i> as per the 16S rDNA sequence analysis. Phylogenetic tree grouped the isolates into four groups, while BOX-PCR fingerprinting allowed further differentiation of the seven isolates. The PGP activity of the isolates was measured on tomato seedlings in plant tissue culture and greenhouse assays. A significant increase in root colonization was observed over 15 days with all the isolates. Greenhouse experiments with these isolates indicated an overall increase in the growth of tomato plants, over 60 days. Isolates TRS-7 and TRS-8 were best plant growth promoters among the seven isolates, with a potential as inoculants to increase tomato productivity.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32904284</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">2405-8440</ISSN><JournalIssue CitedMedium="Print"><Volume>6</Volume><Issue>8</Issue><PubDate><Year>2020</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Heliyon</Title><ISOAbbreviation>Heliyon</ISOAbbreviation></Journal><ArticleTitle>Functional and molecular characterization of plant growth promoting <i>Bacillus</i> isolates from tomato rhizosphere.</ArticleTitle><Pagination><MedlinePgn>e04734</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.heliyon.2020.e04734</ELocationID><Abstract><AbstractText>The rhizosphere offers a quintessential habitat for the microbial communities and facilitates a variety of plant-microbe interactions. Members of the genus <i>Bacillus</i> constitute an important group of plant growth promoting rhizobacteria (PGPR), which improve growth and yield of crops. In a total of 60 bacterial isolates from the tomato rhizosphere, 7 isolates were selected based on distinct morphological characteristics and designated as tomato rhizosphere (TRS) isolates with a number suffixed <i>viz</i>., TRS-1, 2, 3, 4, 5, 7, and TRS-8. All the seven isolates were Gram positive, with <i>in vitro</i> plant growth promoting (PGP) traits like phosphate and zinc solubilization, and also produced indoleacetic acid (IAA), phytase, siderophore, hydrogen cyanide (HCN), and 1-aminocyclopropane-1-carboxylate (ACC) deaminase, besides being antagonistic to other microbes and formed biofilm. The seven isolates belonged to the genus <i>Bacillus</i> as per the 16S rDNA sequence analysis. Phylogenetic tree grouped the isolates into four groups, while BOX-PCR fingerprinting allowed further differentiation of the seven isolates. The PGP activity of the isolates was measured on tomato seedlings in plant tissue culture and greenhouse assays. A significant increase in root colonization was observed over 15 days with all the isolates. Greenhouse experiments with these isolates indicated an overall increase in the growth of tomato plants, over 60 days. 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A" first="Appa Rao" last="Podile">Appa Rao Podile</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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